CUSTOM ITEM: HPLC Column, Bidentate C8 300, 5um, 4.6mm ID x 150mm Length, 300A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑15P‑3M Cogent Bidentate C8™ 300 HPLC Column is a custom‑manufactured, long‑format wide‑pore reversed‑phase column designed for the analysis and separation of macromolecules, including proteins, peptides, polysaccharides, and other high‑molecular‑weight analytes. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, faster equilibration, and extremely robust column lifetime compared with conventional 300 Å silica‑based C8 materials.
The stationary phase uses an aliphatic C8 hydrocarbon chain bonded via direct silicon–carbon bonds to a silica hydride surface. This chemistry creates a phase that is more hydrophobic than traditional wide‑pore C8 columns, yet more balanced than C18 for large biomolecules—providing strong retention without excessive adsorption. The 300 Å pore architecture allows efficient analyte access and mass transfer, which is essential for maintaining peak shape and resolution in macromolecular separations.
Unlike conventional wide‑pore silica phases, the Bidentate C8™ 300 can retain compounds that often show poor retention on traditional C8 columns. It can also retain select hydrophilic macromolecules, particularly in HILIC‑like operating conditions, offering expanded method flexibility. Additionally, the column is stable at very low pH (below pH 2) with no degradation, supporting aggressive analytical methods.
The 4.6 mm ID × 150 mm length format is ideal when maximum resolving power is required for biomolecule separations using UV detection or LC‑MS with a flow splitter. The extended column length increases plate count, improving separation of closely related macromolecular species.
Why Choose the 4.6 × 150 mm Bidentate C8™ 300 Format
This configuration is commonly selected when:
Large biomolecules require 300 Å pore access
Maximum resolution is needed for protein or polysaccharide separations
Standard wide‑pore C8 lacks retention or stability
Wide‑pore C18 causes excessive adsorption or peak broadening
LC‑MS with flow splitting or UV detection is used
The combination of long column length, wide‑pore structure, and TYPE‑C™ silica hydride chemistry provides high resolution and robust performance not achievable with conventional wide‑pore silica columns.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Direct Si–C bonding for exceptional durability
Reduced silanol activity and cleaner peak shapes
Faster equilibration than conventional wide‑pore silica columns
Bidentate C8™ 300 Bonding
Enhanced hydrophobicity vs standard C8
Balanced retention for proteins and macromolecules
Avoids excessive adsorption seen with C18
Wide‑Pore (300 Å) Architecture
Optimized for proteins, peptides, and polysaccharides
Improves mass transfer and peak shape
Enables effective large‑molecule separations
Maximum‑Resolution Analytical Geometry
4.6 mm ID × 150 mm length
High plate count for demanding separations
Compatible with standard analytical HPLC systems
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Supports HILIC‑like operation for select macromolecules
Typical Applications
Protein and peptide analysis (HPLC and LC‑MS)
Polysaccharide separations
Biomolecule characterization
Wide‑pore reversed‑phase chromatography
UV detection workflows and LC‑MS with flow splitting
Low‑pH macromolecule methods
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable